Part:BBa_K3956004:Experience

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Please enter how you used this part and how it worked out.

Applications of BBa_K3956004

This part was used in CCA_San_Diego’s 2021 CollaGene project after a core promoters sequence and directly before the start codon of the ORF, as documented by Decoene et. al, 2019. Although we were able to verify that the construct had been correctly inserted into appropriate selective plates by colony PCR verification, the protein expression was not able to be verified within the time constraints of the competition.

We want to emphasize that this sequence may still be successful, but its efficiency was unable to be demonstrated within the time span that CCA_San_Diego had in the lab. For future teams, we advise testing construct designs with successful expression with and without the sequence to compare expression levels.

User Reviews

UNIQacf7b42072a97d5a-partinfo-00000000-QINU UNIQacf7b42072a97d5a-partinfo-00000001-QINU